Test Bank For Modern Blood Banking & Transfusion Practices 7th Edition By Denise M Harmening Latest Update

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6888_IFC 25/10/18 2:31 PM Page 2 Antigen-Antibody Characteristic Chart* ANTIGENS Antigen System Antigen Name ISBT Name Antigen Freq. % W B RBC Antigen Expression at Birth Antigen Distrib. Plasma/RBC Demonstrates Dosage Antigen Modification Enzyme/Other **D RH1 85 92 strong RBC only no Enz. ↑ **C **E RH2 RH3 68 29 27 22 strong strong RBC only RBC only yes yes Enz. ↑ Enz. ↑ **c **e RH4 RH5 80 98 97 99 strong strong RBC only RBC only yes yes Enz. ↑ Enz. ↑ ce/f Ce Cw RH6 RH7 RH8 64 70 1 92 27 rare strong strong strong RBC only RBC only RBC only no no yes Enz. ↑ Enz. ↑ Enz. ↑ G V RH12 RH10 86 1 92 30 strong strong RBC only RBC only no no Enz. ↑ Enz. ↑ VS RH20 1 32 strong RBC only no Enz. ↑ AET → ZZAP → K KEL1 9 rare strong RBC only occ Enz. → AET+ ↓ ZZAP ↓++ k Kpa KEL2 KEL3 98.8 2 100 rare strong strong RBC only RBC only occ occ Enz. → AET+ ↓ ZZAP ↓++ Enz. → AET+ ↓ ZZAP ↓++ Kpb Jsa KEL4 KEL6 99.9 .01 100 20 strong strong RBC only RBC only occ occ Enz. → AET+ ↓ ZZAP ↓++ Enz. → AET+ ↓ ZZAP ↓++ Jsb †Kx KEL7 — 99.9 99.9 99 99.9 strong weak RBC only RBC low occ occ Enz. → AET+ ↓ ZZAP ↓++ Enz. → AET+ ↓ ZZAP ↓++ FYa FY1 65 10 strong RBC only yes Enz. ↓ AET ↓ ZZAP ↓ FYb FY2 FY3 83 100 23 32 strong strong RBC only RBC only yes no Enz. ↓ AET ↓ ZZAP ↓ Enz. → AET → ZZAP → FY5 100 32 RBC only no Enz. → AET → ZZAP → •FY6 100 32 expressed (on cord cells) expressed (on cord cells) RBC only no Enz. ↓ AET → ZZAP ↓ Rh Kell Duffy *This chart is to be used for general information only. Please refer to the appropriate chapter for more detailed information. AET = 2-aminoethylisthiouronium bromide; ↑ = enhanced reactivity; → = no effect; ↓ = depressed reactivity; occ = occasionally; CGD = chronic granulomatious disease; HDN = hemolytic disease of the newborn; HTR = hemolytic transfusion reaction; NRBC = non-red blood cell; RBC = red blood cell; WBC = white blood cell; ZZAP = dithiothreitol plus papain. • No human antibody to FY6 has been reported. † It has been found that Kx is inherited independently of the Kell system; consequently it is no longer referred to as K15. **Frequency in Asians: D 99%, C 93%, E 39%, c 47%, e 96% 6888_IFC 25/10/18 2:31 PM Page 3 ANTIBODIES Stimulation Serology Saline AHG Comp. Binding Immunoglobin Class IgM IgG Optimum Temperature Clinical Significance HTR HDN RBC occ yes no occ yes warm yes yes RBC RBC/NRBC occ occ yes yes no no occ occ yes yes warm warm yes yes yes yes RBC RBC occ occ yes yes no no occ occ yes yes warm warm yes yes yes yes RBC RBC RBC/NRBC occ occ occ yes yes yes no no no occ occ occ yes yes yes warm warm warm yes yes yes yes yes yes RBC RBC occ occ yes yes no no occ occ yes yes warm warm yes yes yes yes RBC occ yes no occ yes warm yes yes RBC occ yes some occ yes warm yes yes RBC RBC no no yes yes no no rarely no yes yes warm warm yes yes yes yes RBC RBC rarely rarely yes yes no no rarely rarely yes yes warm warm yes yes yes yes RBC RBC no no yes yes no no no occ yes yes warm warm yes yes yes yes RBC rare yes some rare yes warm yes yes RBC RBC rare no yes yes some rarely rare no yes yes warm warm yes yes yes yes RBC no yes ? no yes warm Comments Very rarely IgA anti-D may be produced; however, this is invariably with IgG. Anti-E may occur without obvious immune stimulation. Warm autoantibodies may appear to have anti-e-like specificity. Anti-Cw may occur without obvious immune stimulation. Antibodies to V and VS can present problems in the black population where the antigen frequencies are in the order of 30 to 32. Some antibodies to Kell antigens have been reported to react poorly in low ionic media. Kell system antigens are destroyed by AET and by ZZAP. Anti-K1 has been reported to occur following certain bacterial infections. The lack of Kx expression on RBCs and WBCs has been associated with the McLeod phenotype and CGD. Fya and Fyb antigens are destroyed by enzymes. Fy (a–b–) cells are resistant to invasion by P. vivax merozoites, a malaria-causing parasite. FY3 and 5 are not destroyed by enzymes. FY5 may be formed by interaction of Rh and Duffy gene products. FY6 was described using a monoclonal antibody which reacts with most human red cells except Fy(a–b–). This antigen is responsible for susceptibility of cells to penetration by P. vivax. (Continued on inside back cover) 6888_FM_i-xvi 31/10/18 10:54 AM Page i Modern Blood Banking & Transfusion Practices Seventh Edition Denise Harmening, PhD, MT(ASCP) Director of the Online Masters of Science in Clinical Laboratory Management Professor, Department of Medical Laboratory Science College of Health Sciences Rush University Chicago, Illinois, USA