ASCP MB: Extra-Practice Questions and Correctly Answers Latest 100% PASS

Content Preview

ASCP MB: Extra-Practice Questions and Correct Answers | Latest 2025/2026 | 100% PASS The Klenow fragment of E. coli DNA polymerase I is used: - Answer -to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - Answer -gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucleic acid interaction. Answer -Gel mobility shift assay Which probe is most often used to detect trisomy 21 by interphase FISH? - Answer -alpha satellite DNA probe _____ detect specific chromosomes and numbers of the specific chromosomes. - Answer -Alpha satellite DNA probe Which reagent generates a signal during the annealing stage of a quantitative real time PCR reaction? - Answer -molecular beacon Methylation of cytosine residues is involved in the alteration of gene expression in a number of cancers. Which sequencing technique is best suited to determine if methylation of cytosine residues has taken place in the promoter region of a given gene? - Answer -bisulfite sequencing