CMMB 403 final exam Correctly Answered Latest Update 2025
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CMMB
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CMMB 403 final exam Correctly Answered Latest Update 2025
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| Uploaded on: | May 2, 2025 |
| Last updated: | May 3, 2025 |
| Number of pages: | 45 |
| Written in: | 2025/2026 |
| Type: | Exam (elaborations) |
| Contains: | Questions & Answers |
| Tags: | CMMB 403 final exam Correctly Answered Latest Update 2025 |
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CMMB 403 final exam | Q&A Latest 2025/2026 | 100% PASS what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - Answer -- nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - Answer -endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - Answer -1. non-homologous end joining (NHEJ) - naturally try to repair - imperfect, creates indels causing frameshift mutation 2. directed homology repair - sequence homology on 5' and 3' ends to encourage homologous recombination flanking the double stranded breaks - insert known sequences to try and treat genetic diseases explain the method of conditional gene inactivation - Answer -- make targetting construct in lab
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