CMMB 411 Final Q&A Latest Update 2025
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CMMB
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CMMB
CMMB 411 Final Q&A Latest Update 2025
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| Uploaded on: | May 2, 2025 |
| Last updated: | May 12, 2025 |
| Number of pages: | 56 |
| Written in: | 2025/2026 |
| Type: | Exam (elaborations) |
| Contains: | Questions & Answers |
| Tags: | CMMB 411 Final Q&A Latest Update 2025 |
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CMMB 411 Final | Q&A Latest 2025/2026 | 100% PASS What is the Hi-C method? How do you do it? High-throughput chromosome conformation capture: Basically allows us to see what sequences are spatially close (not necessarily close in sequence). 1. Cross-link spatially close DNA w formaldehyde 2. digest using restriction fragments and fill in sticky ends with biotin labeled bases 3. turn fragments into circular DNA (blunt end ligation) 4. digest (shear) the DNA 5. Used labeled beads to isolate biotin labeled junctions 6. Analyze junctions in Hi-C analysis to map genome-wide interactions What is WGBS? How do you do it? Whole genome bisulfite sequencing: Allows us to determine where C is methylated and thus gives us insight into epigenetics. 1. Add bisulfite which converts non-methylated C into U.
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